Jessica Cannavino - Bottinelli

CURRICULUM VITAE ET STUDIORUM

Personal details

Date and place of birth: 15 November 1984, Pavia, Italy
Citizenship: Italian
Languages: Italian (mother language)
English (high level written and spoken)
French (good level written and spoken) Spanish (good written and spoken)
Address: University of Pavia
Department of Molecular Medicine, via Forlanini 6 Pavia, 27100
Mobile: +393495593923
Email: jessica.cannavino@gmail.com  -  jessica.cannavino@unipv.it

Education and training

February 2013: PhD degree in Physiology and Neuroscience. Title of thesis: Molecular mechanisms underlying skeletal muscle disuse atrophy in human and animal models.
November 2009-November 2012: PhD student in Prof Bottinelli’s laboratory at University of Pavia, Department of Molecular Medicine.
September 2008: Bachelor of science in Biomedical Biology vote: 110/110 with honour. Title of thesis Functional dissection of a human origin of replication
September 2007 – September 2008: Internship in Dr Fiorenzo Peverali’s laboratory, C.N.R, Pavia.
December 2006: Bachelor of science in Biology vote 106/110 title of thesis: Nicotine effects on calcium concentration in smooth muscular cells
January 2006 – July 2006: Internship in Prof. Franco Tanzi laboratory in University of Pavia, Department of Biology.

Courses

14-18 June: Muscle Science summer school, Berlin.

Research Experience

PhD Research: During my PhD I was focused on studying skeletal muscle adaptation to disuse condition, and on clarifying the molecular mechanisms causing disuse muscle atrophy both in human and animal.
For this purpose three skeletal muscle atrophy models were used: the hindlimb unloaded mice animal model, the Bed Rest and unilateral lower limb suspension human models; results from hindlimb animal model and Bed Rest human model are enclosed in my PhD thesis “Molecular mechanisms underlying skeletal muscle disuse atrophy in human and animal models”.
Bachelor research: DNA replication initiates by recruiting the replication machinery to thousands of sites, termed replication initiation sites or replication origins (Ori), scattered on chromosomes. The Lamin B2 origin of DNA replication (LamB2-ori) is confined to a site mapped on the chromosome 19p.13.3. We showed that a 1.2kb fragment of the ori initiates replication from random ectopic integration sites of the human genome. To perform a detailed mutagenesis analysis on the 1.2kb ori fragment, we have applied recombinase mediated cassette exchange (RMCE) that allows to compare the replication activity of different ori mutants in the same chromosomal context. Results indicate that a fragment of LamB2-ori shorter than 300bp maintains the ability to initiate replication.

Laboratory Skills

DNA and RNA extraction
primers design
semi-quantitative PCR and REAL TIME PCR
Agarose gel electrophoresis
One dimensional electrophoresis
Two dimensional electrophoresis
Transformation of Escherichia coli
Stable and transient trasfection in human cell
plasmid extraction
Site specific recombinase (RMCE)
HeLa and C2C12 cell culture
proteins quantification
DNA cloning
Enzyme digestion
Luciferase assay
Electrophoresis to separate myofibrillar protein isoforms
Western Blotting
Muscles dissection
Histochemistry staining
Immunohistochemistry

Publications

The time course of the adaptations of human muscle proteome to bed rest and the underlying mechanisms.
Brocca L, Cannavino J, Coletto L, Biolo G, Sandri M, Bottinelli R, Pellegrino MA.
J Physiol. 2012 Oct 15;590(Pt 20):5211-30.

Oral Presentations

ANNUAL MEETING OF YOUNG RESEARCH IN PHYSIOLOGY Sestri Levante (GE)
30 May -1 June 2012
Presentation: ”Redox imbalance and metabolic impairment in skeletal muscle of hindlimb unloaded mice: a time course study”
VIII MEETING IIM: Sestri Levante (GE), 27-29 October 2011
Presentation: “The time course of disuse atrophy in a slow and fast muscle of hindlimb unloaded mice”
62° ITALIAN SOCIETY OF PHYSIOLOGY Sorrento (NA) 25-27 September 2011
Presentation: “The time course of disuse atrophy in a slow and fast muscle of hindlimb unloaded mice”
EUROPEAN SOCIETY FOR MUSCLE RESEARCH Berlin 14-18 September 2011
Presentation: “The time course of disuse atrophy in a slow and fast muscle of hindlimb unloaded mice”
BIOCHEMISTRY AND PHYSIOLOGY PhD MEETING Sondrio 10-12 June 2011
Presentation: “Skeletal muscle adaptations to a decrease in neuromuscular activity”
VII MEETING IIM Siena 14-16 October 2010
Presentation: “ACUTE AND CHRONIC EXPOSURE TO BRANCHED CHAIN AMINOACID ENRICHED MIXTURE ACTIVATES BASAL AND CONTRACTION MEDIATED MTOR/P70 SIGNALING IN SOLEUS MUSCLE OF ELDERLY RATS.

Posters presentation

V ISSBB Padua 17-19 November 2011
Poster: “The time course of disuse atrophy in a slow and fast muscle of hindlimb unloaded mice”
16th SFRR-INTERNATIONAL CONFERENCE London 5-9 September 2012
Poster:”Redox imbalance in slow and fast muscle of hindlimb unloaded mice study”

AWARDS

SFRRI YOUNG INVESTIGATOR AWARD 16th SFFR-international Conference London 5-9 September 2012.