Manuela Maffei - Bottinelli

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Manuela Maffei

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Curriculum Vitae

PERSONAL INFORMATIONS

Name
 Manuela Maffei
e-mail
 Manuela.maffei@unipv.it
Nationality
 Italian

EDUCATION AND TRAINING

Title of qualification awarded
  High school specializing in scientific subjects
• Dates  1992 – 1997
• Name and type of organization providing education and training  Scientific High School "A. Omodeo" in Mortara  (Italy)

Degree  
Biological Sciences
           Title of the Thesis  "Structure and folding dynamics of human
β2-microglobulin.Pathological and Physiological implication"
• Dates  1997 – 2002
• Name and type of organization  providing education and training  University of Study of Pavia, Pavia (Italy)
Graduate school
in Applied Genetics
           Title of the Thesis ": "Chimeric adeno-associated virus/antisense U1 small nuclear RNA effectively rescues dystrophin synthesis and muscle function by local treatment of mdx mice.
• Dates  2002-2005
• Name and type of organization  providing education and training  University of Study of Pavia, Pavia (Italy)

PhD
Physiological Science
      Title of the Thesis:  : "Molecular mechanism of skeletal myosin isoforms diversity"  
• Dates  2005-2009
• Name and type of organization  providing education and training  University of Study of Pavia, Pavia (Italy)

WORK EXPERIENCES


• Company
University of the Study of Pavia

FROM FEBRUARY 2002 TO NOW

• Description


Since 2003 I have been engaged in research projects involving the study of the plasticity of muscle tissue in different physiological and pathological conditions (aging, exercise, muscle disuse, muscular dystrophy, diabetes) and  most of my research activity is on  the study of the acto-myosin interaction and on the study of the molecular determinants of the functional differences among skeletal myosin isoforms. Most work  done is to improve techniques to study the functional properties of single molecule: extraction of pure myosin isofoms from single muscle fibers; in vitro motility assay (IVMA) and manipulation of single molecules by laser trap (optical trap or OT).
The studies were performed on human muscle biopsies or on samples from animal models; biopsies were functionally characterized, through in vivo and in vitro evaluations of force and speed of muscle contraction, and structurally through immunohistochemical analysis of tissue.

Experimental works led to the publication of 6 scientific papers in international journals.

Finally I have been engaged in teaching activity at the University of Pavia, I held seminars and I have participated to national and international conferences always making a scientific contribution with data presentation both as oral communications and as posters


OTHER EXPERIENCES


• In AY 2010/2011 I have been engaged in teaching activity as an Adjunct Professor of Physiology in the degree of Health and Techniques Professions at the University of Pavia

• With the research activity I have done Tutoring activity of Human Physiology for the degree course in Nursing and Obstetrics Science, University of Pavia (AY from AY 2006/07 2009/2010)

• In November 2010 I did an Internship at the School of Physiology and Biophysics 2010 the course was held at the Department of physic, University of Florence, director of the school Prof. G. Fano, Head Professor of the course. Prof. C. Reggiani.




SCIENTIFIC PAPERS


Ultrafast force-clamp spectroscopy of single molecules reveals load dependence of myosin working stroke.  
Capitanio M,  Canepari  M,  Maffei  M, Beneventi D, Monico C, Vanzi F, Bottinelli R, Pavone FS.  
Nat Methods. 2012 Oct;9(10):1013-9. doi: 10.1038/nmeth.2152. Epub 2012 Sep 2.  


Actomyosin kinetics of pure fast and slow rat myosin isoforms studied by in vitro motility assay approach.  
Canepari  M,  Maffei  M, Longa E, Geeves M, Bottinelli R.  
Exp Physiol. 2012 Jul;97(7):873-81. doi: 10.1113/expphysiol.2012.064576. Epub 2012 Mar 30.  

Actin sliding velocity on pure myosin isoforms from dystrophic mouse muscles.  
Canepari  M, Rossi R, Pansarasa O,  Maffei  M, Bottinelli R.  
Muscle Nerve. 2009 Aug;40(2):249-56. doi: 10.1002/mus.21302.  

New techniques in linear and non-linear laser optics in muscle research.  
Vanzi F, Capitanio M, Sacconi L, Stringari C, Cicchi R,  Canepari  M,  Maffei  M, Piroddi N, Poggesi C, Nucciotti V, Linari M, Piazzesi G, Tesi C, Antolini R, Lombardi V, Bottinelli R, Pavone FS.  
J Muscle Res Cell Motil. 2006;27(5-7):469-79. Epub 2006 Aug 24. Review.
PMID:  

Two independent mechanical events in the interaction cycle of skeletal muscle myosin with actin.  
Capitanio M,  Canepari  M, Cacciafesta P, Lombardi V, Cicchi R,  Maffei  M, Pavone FS, Bottinelli R.  
Proc Natl Acad Sci U S A. 2006 Jan 3;103(1):87-92. Epub 2005 Dec 21.  

Temperature dependence of speed of actin filaments propelled by slow and fast skeletal myosin isoforms.  
Rossi R,  Maffei  M, Bottinelli R,  Canepari  M.  
J Appl Physiol. 2005 Dec;99(6):2239-45. Epub 2005 Aug 11.



LANGUAGE SKILLS

                                
Mother tongue:
Italian

English

• Reading skills  EXCELLENT
• Writing skills  EXCELLENT
• Speaking skills  GOOD

COMPUTER SKILLS  

Operating system: Windows 2000, XP, Vista, Windows7
Software: Word, Excel, Power Point, Adobe Photoshop CS3, Acrobat Reader 6.0, Graphpad Prism 5, Image Master Platinum, Scion Image Stat View 5.1
Internet Explorer, Microsoft Outlook.



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