Roberto Bottinelli - Bottinelli

Roberto Bottinelli
Short biographical sketch

BORN: 20 April 1956, Pavia, Italy.
ADDRESS: Dipartimento di Medicina Molecolare, Sezione di Fisiologia umana, Via Forlanini 6, 27100 Pavia, Italy; Tel: +39.0382987257; fax +39.0382.987664; e-mail: roberto.bottinelli@unipv.it
RESEARCH FIELD: Muscle physiology: the cellular and molecular mechanisms at the basis of the structural and functional heterogeneity and plasticity of skeletal muscle in health and disease
RESEARCH ACTIVITY: He devoted most of his research activity to the study of the cellular and molecular determinants of skeletal muscle structural and functional plasticity. The most recent activity relate to three strictly related topics: (i) the mechanisms of the functional diversity among skeletal myosin isoforms studied at molecular level and at the level of the kinetics of acto-myosin interaction; (ii) the impact of physiologic and pathologic conditions on structure and function of skeletal muscle and the underlying cellular and molecular mechanisms; (iii) the impact of gene, cell and pharmacological therapies on skeletal muscle of animal models of muscular dystrophies. All studies have been performed through a wide network of national and international collaborations.
The results of his research work have been published in 99 publications in journal indexed in PUB MED and with impact factor; total impact factor 645, mean 6.52; H index 37 ISI, 34 Scopus (from 1996), 41 Google Scholar; citations: 4736 (ISI). Google Scholar web page: http://scholar.google.it/citations?user=FSflFosAAAAJ.
The most important works were published in Nature (1984, 2006 (2)), Nat Med (2006), Nature Methods (2012), J Clin Invest (2004, 2006), EMBO Journal (1997), Proc Natl Acad Sci USA (2006 (2); 2007), Circulation Research (1984, 1998), Journal of Physiology (London), the leading physiology journal (1991, 1994 (2), 1996 (2), 1997, 2003 (2), 2004, 2006, 2007, 2009, 2010 (1), 2011 (2), 2012), Science (2003).
EDITORIAL ACTIVITY: Reviewing Editor of the Journal of Physiology (London) (2004-2006); Senior Editor of the Journal of Physiology (London) (2006-2011); member of the executive committee of the Journal of Physiology (2007-2011); Senior Editor European Journal of Applied Physiology (2003-2010); member of F1000 since 206; Associate Editor Frontiers in Skeletal Muscle Physiology (2010).
MEMBERSHIPS
Physiological Society (UK)
Biophysical Society (USA)
Italian Physiological Society (IT)
American College of Sport Medicine (USA)
MOST IMPORTANT ONGOING COLLABORATIONS:
M. Geeves, University of Kent, UK: kinetics of acto-myosin interactions in skeletal myosin isoforms
F. Pavone and M. Capitanio, LENS, Florence: single molecule mechanics of skeletal myosin isoforms
C. Reggiani, Padova: intracellular signaling during skeletal muscle adaptations
G. Cossu, University of Milano: cell therapy for Muscular Dystrophies
Diana Conte Camerino, University of Bari, Italy
MAJOR RESEARCH FOUNDING:
European Community (V Framework): Pan European Network for Ageing Muscle - Better Ageing 2001-2005.
Telethon Foundation: "Analysis of functional impairment of skeletal muscle in murine models of muscular dystrophy and of functional recovery following mesoangioblast treatments" (2003-2006)
Cariplo Foundation: "Studio di un protocollo terapeutico per correggere le distrofie muscolari attraverso l’uso di un nuovo tipo di cellula staminale" (2004-2006); "Miglioramento dell’efficacia clinica di un nuovo tipo di cellula staminale, i mesoangioblasti, nel modello pre-clinico della distrofia di Duchenne"  (2007-2009); "La miopatia steroidea: caratterizzazione molecolare, istopatologica ed elettrofisiologica" (2011-2013)
Italian Space Agency: "The cellular and molecular mechanisms of skeletal muscle plasticity in disuse induced atrophy and in pathologic conditions" (OSMA project, 2006-2009)
European Community (VII Framework): MYOAGE (2009-2012)
Current position title
Full Professor of Physiology, Medical School, University of Pavia, Italy
Educational background
University of Pavia, Pavia, Italy, M.D. 1981
University of Pavia, Specialization in Sport Medicine, 1986
University of Pavia, Ph.D. Physiology 1988
List of 10 major publications

Capitanio M, Canepari M, Maffei M, Beneventi D, Monico C, Vanzi F, Bottinelli R & Pavone FS. (2012). Ultrafast force-clamp spectroscopy of single molecules reveals load dependence of myosin working stroke. Nature methods 9, 1013-1019.

Pellegrino MA, Desaphy JF, Brocca L, Pierno S, Camerino DC & Bottinelli R. (2011). Redox homeostasis, oxidative stress and disuse muscle atrophy. The Journal of physiology 589, 2147-2160.

Sampaolesi M, Blot S, D'Antona G, Granger N, Tonlorenzi R, Innocenzi A, Mognol P, Thibaud JL, Galvez BG, Barthelemy I, Perani L, Mantero S, Guttinger M, Pansarasa O, Rinaldi C, Cusella De Angelis MG, Torrente Y, Bordignon C, Bottinelli R & Cossu G. (2006). Mesoangioblast stem cells ameliorate muscle function in dystrophic dogs. Nature 444, 574-579.

D'Antona G, Lanfranconi F, Pellegrino MA, Brocca L, Adami R, Rossi R, Moro G, Miotti D, Canepari M & Bottinelli R. (2006). Skeletal muscle hypertrophy and structure and function of skeletal muscle fibres in male body builders. J Physiol 570, 611-627.

Capitanio M, Canepari M, Cacciafesta P, Lombardi V, Cicchi R, Maffei M, Pavone FS & Bottinelli R. (2006). Two independent mechanical events in the interaction cycle of skeletal muscle myosin with actin. Proc Natl Acad Sci U S A 103, 87-92.

Sampaolesi M, Torrente Y, Innocenzi A, Tonlorenzi R, D'Antona G, Pellegrino MA, Barresi R, Bresolin N, De Angelis MG, Campbell KP, Bottinelli R & Cossu G. (2003). Cell therapy of alpha-sarcoglycan null dystrophic mice through intra-arterial delivery of mesoangioblasts. Science 301, 487-492.

D'Antona G, Pellegrino MA, Adami R, Rossi R, Carlizzi CN, Canepari M, Saltin B & Bottinelli R. (2003). The effect of ageing and immobilization on structure and function of human skeletal muscle fibres. J Physiol 552, 499-511.

Bottinelli R & Reggiani C. (2000). Human skeletal muscle fibres: molecular and functional diversity. Prog Biophys Mol Biol 73, 195-262.

Bottinelli R, Canepari M, Pellegrino MA & Reggiani C. (1996). Force-velocity properties of human skeletal muscle fibres: myosin heavy chain isoform and temperature dependence. J Physiol 495 ( Pt 2), 573-586.

Bottinelli R, Schiaffino S & Reggiani C. (1991). Force-velocity relations and myosin heavy chain isoform compositions of skinned fibres from rat skeletal muscle. J Physiol 437, 655-672.

Previous employment (in chronological order)
1992-1998   Researcher in Physiology, Faculty of Medicine, University of Pavia
1998-2001   Associate Professor of Physiology, Faculty of Medicine, University of Pavia
2001-   Full Professor of Physiology, , Faculty of Medicine, University of Pavia

Experience (in chronological order)
Teaching:
The full Physiology course for Medical students since 1996
A Physiology course for Motoric Sciences degree since 1999
The Physiology Course for Medical and Pharmaceutical Biotechnology Degree since 2004
The Physiology Course in English for Medical students since 2010
Administrative:
Head of the Department of Experimental Medicine, University of Pavia, 2005-2008
Head of the Department of Physiology, University of Pavia, 2008-2011
Director of the Interuniversity Institute of Myology (2008-2011)
Director of the Specialization school of Sport Medicine for medical doctors 2002-
Research:
P.I. of the Muscle Biophysics Laboratory of the Department since 1999
Experimental:
1981-1988   Cardiac physiology: factors affecting contractility, relaxation, intracellular calcium kinetics
1982-1986   contractility of intact isolated frog muscle fibres
1984-   muscle fibre types, myosin and myofibrillar protein isoforms and their contractile and energetic properties
1998-   analysis of myosin motor function at single molecule level by reconstituted contractile systems in vitro: in vitro motility assays and optical trap
2002-2007  study of cellular, gene and pharmaceutical therapies of muscular dystrophies
2002-   proteomic analysis of muscle phenotype adaptations
2009-  gene expression analysis and analysis of intracellular pathways underlying skeletal muscle plasticity in health and disease

Publications in extenso last three years: 2009-2011

Tedesco FS, Gerli MF, Perani L, Benedetti S, Ungaro F, Cassano M, Antonini S, Tagliafico E, Artusi V, Longa E, Tonlorenzi R, Ragazzi M, Calderazzi G, Hoshiya H, Cappellari O, Mora M, Schoser B, Schneiderat P, Oshimura M, Bottinelli R, Sampaolesi M, Torrente Y, Broccoli V & Cossu G. (2012). Transplantation of Genetically Corrected Human iPSC-Derived Progenitors in Mice with Limb-Girdle Muscular Dystrophy. Sci Transl Med 4, 140ra189.

Porcelli S, Marzorati M, Pugliese L, Adamo S, Gondin J, Bottinelli R & Grassi B. (2012). Lack of functional effects of neuromuscular electrical stimulation on skeletal muscle oxidative metabolism in healthy humans. J Appl Physiol.

Farini A, Sitzia C, Navarro C, D'Antona G, Belicchi M, Parolini D, Del Fraro G, Razini P, Bottinelli R, Meregalli M & Torrente Y. (2012). Absence of T and B lymphocytes modulates dystrophic features in dysferlin deficient animal model. Exp Cell Res 318, 1160-1174.

D'Antona G, Mascaro A, Monopoli A, Miglietta D, Ongini E & Bottinelli R. (2012). Nitric oxide prevents atorvastatin-induced skeletal muscle dysfunction and alterations in mice. Muscle Nerve.

Capitanio M, Canepari M, Maffei M, Beneventi D, Monico C, Vanzi F, Bottinelli R & Pavone FS. (2012). Ultrafast force-clamp spectroscopy of single molecules reveals load dependence of myosin working stroke. Nature methods 9, 1013-1019.

Canepari M, Maffei M, Longa E, Geeves M & Bottinelli R. (2012). Actomyosin kinetic of pure fast and slow rat myosin isoforms studied by in vitro motility assay approach. Exp Physiol.

Brocca L, Cannavino J, Coletto L, Biolo G, Sandri M, Bottinelli R & Pellegrino MA. (2012). The time course of the adaptations of human muscle proteome to bed rest and the underlying mechanisms. J Physiol 590, 5211-5230.

Tedesco FS, Hoshiya H, D'Antona G, Gerli MF, Messina G, Antonini S, Tonlorenzi R, Benedetti S, Berghella L, Torrente Y, Kazuki Y, Bottinelli R, Oshimura M & Cossu G. (2011). Stem cell-mediated transfer of a human artificial chromosome ameliorates muscular dystrophy. Sci Transl Med 3, 96ra78.

Pellegrino MA, Desaphy JF, Brocca L, Pierno S, Camerino DC & Bottinelli R. (2011). Redox homeostasis, oxidative stress and disuse muscle atrophy. The Journal of physiology 589, 2147-2160.

Maffiuletti NA, Minetto MA, Farina D & Bottinelli R. (2011). Electrical stimulation for neuromuscular testing and training: state-of-the art and unresolved issues. European journal of applied physiology 111, 2391-2397.

Gondin J, Brocca L, Bellinzona E, D'Antona G, Maffiuletti NA, Miotti D, Pellegrino MA & Bottinelli R. (2011). Neuromuscular electrical stimulation training induces atypical adaptations of the human skeletal muscle phenotype: a functional and proteomic analysis. J Appl Physiol 110, 433-450.

Camerino GM, Pellegrino MA, Brocca L, Digennaro C, Camerino DC, Pierno S & Bottinelli R. (2011). Statin or fibrate chronic treatment modifies the proteomic profile of rat skeletal muscle. Biochemical pharmacology 81, 1054-1064.

Bottinelli R & Westerblad H. (2011). Reactive oxygen and nitrogen species in skeletal muscle: acute and long-term effects. The Journal of physiology 589, 2117-2118.

Tricarico D, Mele A, Camerino GM, Bottinelli R, Brocca L, Frigeri A, Svelto M, George AL, Jr. & Camerino DC. (2010). The KATP channel is a molecular sensor of atrophy in skeletal muscle. J Physiol 588, 773-784.

Flati V, Caliaro F, Speca S, Corsetti G, Cardile A, Nisoli E, Bottinelli R & G DA. (2010). Essential amino acids improve insulin activation of AKT/MTOR signaling in soleus muscle of aged rats. Int J Immunopathol Pharmacol 23, 81-89.

Desaphy JF, Pierno S, Liantonio A, Giannuzzi V, Digennaro C, Dinardo MM, Camerino GM, Ricciuti P, Brocca L, Pellegrino MA, Bottinelli R & Camerino DC. (2010). Antioxidant treatment of hindlimb-unloaded mouse counteracts fiber type transition but not atrophy of disused muscles. Pharmacol Res 61, 553-563.

D'Antona G, Ragni M, Cardile A, Tedesco L, Dossena M, Bruttini F, Caliaro F, Corsetti G, Bottinelli R, Carruba MO, Valerio A & Nisoli E. (2010). Branched-chain amino acid supplementation promotes survival and supports cardiac and skeletal muscle mitochondrial biogenesis in middle-aged mice. Cell Metab 12, 362-372.

Canepari M, Pellegrino MA, D'Antona G & Bottinelli R. (2010). Skeletal muscle fibre diversity and the underlying mechanisms. Acta Physiol (Oxf) 199, 465-476.

Canepari M, Pellegrino MA, D'Antona G & Bottinelli R. (2010). Single muscle fiber properties in aging and disuse. Scand J Med Sci Sports 20, 10-19.

Brocca L, Pellegrino MA, Desaphy JF, Pierno S, Camerino DC & Bottinelli R. (2010). Is oxidative stress a cause or consequence of disuse muscle atrophy in mice? A proteomic approach in hindlimb-unloaded mice. Exp Physiol 95, 331-350.

Borina E, Pellegrino MA, D'Antona G & Bottinelli R. (2010). Myosin and actin content of human skeletal muscle fibers following 35 days bed rest. Scand J Med Sci Sports 20, 65-73.