Valentina Agoni - Bottinelli

University of Pavia
Viale Argonne 8, PV 27100
Phone:328-8352871
Email:valentina.agoni01@ateneopv.it

Personal

Born on August 13, 1985.
Italy Citizen.

Education

B.S. in Molecular Biology, Pavia University, 2008.
Dissertation title: Cell death induction and nitric oxide biosynthesis in white poplar (Populus alba)
suspension cultures exposed to alfalfa saponins.

M.A. in Sperimental and Applied Biology, Pavia University, 2009.
Dissertation title: Identification and characterization of MDR (Multidrug Resistant) Pseudomonas aerug-
inosa from clinical isolates.

Research experience and acquired methods

2007-08: Laboratory of Plant Biotechnology (Prof. Carbonera)
Research themes: PCD (Programmed Cell Death) processes and the NO (Nitric Oxide) biosynthesis in
poplar cell coltures exposed to saponins.

2010: Hospital "San Matteo", Pavia (Dr. Marone)
Research themes: Identification of pathogens (oxidase test, catalase test, coagulase test, API tests,
immunological methods, Phoenix), antibiogram, serum bacteriocidal test.

2010: Laboratory of Molecular Microbiology (Prof. Riccardi and Prof. Pasca)
Research themes: Mechanisms of bacterial resistance (in particular: efflux pumps inhibitors, mutations
of genes coding fluoroquinolones targets, biofilm).

2010-11: Laboratory of Proteomics (Prof. Bottinelli, Prof.ssa Pellegrino)
Research themes: Cellular and molecular mechanisms at the basis of the structural and functional
heterogeneity and plasticity of skeletal muscle in health and disease.

Acquired methods

- PCR (Polymerase Chain Reaction)
- Electrophoresis
- 2D Electrophoresis
- Nucleic acids extraction
- Nucleic acids purification
- Real-Time PCR
- RAPD (Rapid Amplification of Polymorphic DNA)
- Bacterial transformation
- Cloning
- Plant cells cultures
- Identification of pathogens (bacteria and fungi) from different clinical isolates
- Phoenix
- Spectrophotometric assays
- Enzyme assays

Foreign languages

English (intermediate)
French (intermediate)

Computer skills

Support programs: Office package, many browsers
Networking (including NCBI website)

Publications

Balestrazzi A, Agoni V, Tava A, Avato P, Biazzi E, Raimondi E, Macovei A, Carbonera D., Cell death induction
and nitric oxide biosynthesis in white poplar (Populus alba) suspension cultures exposed to alfalfa saponins, Physiol
Plant. 2011 Mar;141(3):227-38. 2010 Dec 30.