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  RESEARCH ACTIVITY > Extracellular matrix and Inflammation, Messina  
 
 
 
Extracellular matrix and Inflammation, Messina
 
 
 
 
Research Group

Affiliations
University of Messina
Department of Clinical and Experimental medicine
Policlinico Universitario, Torre biologica p.5, Via Consolare Valeria, 98125 Messina Italy
University of Messina
Department of Biomedical and Dental Sciences and Morphofunctional Images
Policlinico Universitario, Torre biologica p.5, Via Consolare Valeria, 98125 Messina
http://www.unime.it/

Background and specific aims

The scientific researches of this team are focused on the

physiopathological study of the glycosaminoglycans into the more common human pathologies and

in pathological experimental models in animals.

The group has been set up several pathological experimental models in rodents such as acute cirrhosis, rheumatoid arthritis, hepatic fibrosis and pancreatitis in order to study and to evaluate the effective role of glycosaminoglycans with the aim to apply these studies in humans.

The involvement of glycosaminoglycans and their antioxidant activity is also studied on human skin

fibroblast cultures and the evaluation of glycosaminoglycans interactions with MMPs and TIMPs.

The direct action of the glycosaminoglycans of transcription factors such as NF-kB

and initiators of apoptosis such as caspases was also assessed. Other research has been conducted on the increased synthesis of hyaluronic acid, in fibroblast culture medium after treatment with certain growth factors and cytokines, and the synergic action exerted by oxidative stress. Several experiments to assess the role of hyaluronic acid, according to its molecular weight, in normal cells, as well as in cells treated with pro-inflammatory agents  have been conducted in order to evaluate its modulatory effects exerted througt both the receptors CD44 and TLRs. With particular interest we investigated the role of glycosaminoglycans, hyaluronic acid especially, in experimental arthritis, both in vivo and in vitro together with all the mediators related to the inflammatory process.
Research projects

Currently the following studies are in progress: 1)The active role of SER-glycine in neurodegenerative processes and experimental arthritis; 2)The involvement of proteoglycans decorin and biglycan in inflammatory processes of cartilage; 3) The pro-inflammatory action of biglycan in different type cell cultures; 4)The role and activities of miRNAs in cells exposed to inflammatory agents derived from the cartilage.

Facilities and equipment

Basic Instrumentation for the biochemical laboratory – Oligo-sequencer analyzer –  Incubators – Basic PCR and  RealTime PCR – Cellular micromanipulator - Capillary electrophoresis – HEPA and laminar flow hoods - CO2 incubators - Microplate reader - UV-VIS readers - Apparatus for western blot.


Recent pubblications
6-MER HYALURONAN OLIGOSACCHARIDES MODULATE NEUROINFLAMMATION AND ALPHA SYNUCLEIN EXPRESSION IN NEURON-LIKE SH-SY5Y CELLS - Scuruchi M, D'Ascola A, Avenoso A, Campana S, Abusamra YA, Spina E, Calatroni A, Campo GM, Campo S. - J Cell Biochem. May 11. doi: 10.1002/jcb.25595 ( 2016 )
INHIBITION OF SMALL HA FRAGMENT ACTIVITY AND STIMULATION OF A2A ADENOSINE RECEPTOR PATHWAY LIMIT APOPTOSIS AND REDUCE CARTILAGE DAMAGE IN EXPERIMENTAL ARTHRITIS - Campo GM, Micali A, Avenoso A, D'Ascola A, Scuruchi M, Pisani A, Bruschetta A, Calatroni A, Puzzolo D, Campo S. - Histochem Cell Biol. 143:531-43 ( 2015 )
BETA-ARRESTIN 1 IS INVOLVED IN THE CATABOLIC RESPONSE STIMULATED BY HYALURONAN DEGRADATION IN MOUSE CHONDROCYTES - Campo GM, Avenoso A, D'Ascola A, Scuruchi M, Calatroni A, Campo S. - Cell Tissue Res. 361:567-579 ( 2015 )

 
 
 
 
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Last update: january 08th 2017 h 15:04
 
 
 
 
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