| Following isolation from the ovaries, antral oocytes (a) were stained with hoechst 3342 and classified into NSN (b1) and SN (b2) oocytes on the basis of their chromatin organisation. Following in vitro maturation, insemination and embryo culture, only SN oocytes were capable of reaching the 4-cell stage and developing to blastocyst (e2). In contrast NSN oocytes were blocked at the 2-cell stage (c1). A high percentage of SN-derived blastocysts developed further to reach the hatching stage (f2). (modified from Zuccotti et al., 2002) |
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