| Through series of confocal images of the nucleus of an isolated growing oocyte (40-50 um in diameter) reacted with CREST antibodies and counterstained with Hoechst (a). The bright punctate dots indicate centromeres. Positions of centromeres depicted in b-g relative to surface of the nucleolus. Arrowheads indicate two groups of three centromeres each localised on the oposite sides of the nucleolus. (h) Stack reconstruction of the whole nucleus made with NIH image. (i) 3D reconstruction of the position of centromeres (green dots) within the oocyte nucleus relative to the nucleolus. Bar: 5 um. |
Confocal images of NSN (a-k) and SN (m-w) oocytes reacted with CREST antibody. The bright punctate dots indicate the centromeres. The 3D reconstruction clearly shows that fewer centromeres are closely associated with the nucleolus of NSN (l) in comparison to the nucleolus of SN (x) oocytes. Bar: 5 um. |
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